Morphological features of apoptosis pdf
Paraapoptosis or nonclassical apoptosis is a specific morphological type of non necrotic cell death 1618 and is characterized by cytoplasmic vacuolization, condensed chromatin (but not early margination of the chromatin) and swollen mitochondria.A 4 Apoptosis, Cell Death, and Cell Proliferation Manual Table 1: Differential features and significance of necrosis and apoptosis. Necrosis Apoptosis Morphological features Loss of membrane integrity Membrane blebbing, but no loss of integrity morphological features of apoptosis pdf
MORPHOLOGICAL AND BIOCHEMICAL CHARACTERIZATION AND ANALYSIS OF APOPTOSIS 221 The methods described below take advantage of the most important morphological and biochemical changes occurring in apoptotic cells. The morphological criteria for apoptosis have been detailed previously and will be emphasized under the technique of microscopy.
These forms of cell death that have certain morphological features of both necrosis and apoptosis have been given the term aponecrosis (Formigli et al. , 2000). By affecting the mitochondrial respiratory chain with antimycin A, Formigli and coworkers induced a type of cell death that shared dynamic, molecular, and morphological features with both apoptosis and necrosis. Confirmation of apoptosis is then based on other criteria, such as demonstration of the internucleosomal DNA fragmentation in electrophoresis or analysis of the morphological features. Electrophoresis does not specify the cell types undergoing apoptosis and it is not sensitive enough to morphological features of apoptosis pdf The similarity of the biochemical events during apoptosis in different situations is reflected by a high uniformity of morphological changes in many situations
Apoptosis is characterised by a series of typical morphological features, such as shrinkage of the cell, fragmentation into membranebound apoptotic bodies and rapid phagocytosis by neighbouring morphological features of apoptosis pdf Morphological Features of Organelles during Apoptosis: An Overview Maria Grazia Bottone 1, 2, Giada Santin 1, 2, Francesca Aredia 2, Graziella Bernocchi 1, chemical events during apoptosis in different situations is reflected by a high uniformity of morphological changes in many situations of naturally occurring or experimentally induced cell death. The unifying concept of apoptosis has been derived from the observation of this morphological consistency of dying cells almost 30 years ago. May 07, 2013 In apoptosis, cell death is genetically regulated in response to a variety of stimuli and, in vivo, cells are rapidly removed by phagocytes without major perturbation of the tissue integrity. In contrast to necrosis, apoptosis is a regulated, energydependent form of cell death leading to phagocytosis of cellular remnants by neighboring cells. Characteristic morphological features of these two forms of cell death will be discussed and correlated to underlying molecular mechanisms.Rating: 4.92 / Views: 738